| 초록 |
We have developed a new synergistically integrated nanohybrid consisting of Fe3O4 magnetic nanoparticles (MNPs) and platinum nanoparticles (Pt NPs), simultaneously immobilized on the surface of graphene oxide (GO). And we have formed antibody-conjugated nanohybrids, which are expected to efficiently bind target cancer cells. Application of H2O2 and the selected peroxidase substrate, 3,3´, 5,5´-tetramethylbenzidine (TMB) to the nanohybrid then promotes oxidation of TMB to generate a blue product. The constructed nanohybrid displayed up to 30 times higher maximal reaction velocity (Vmax) compared to that of free GO, and enabled rapid detection of target cancer cells. Specifically, using this new assay system, we were able to detect clinically important breast cancer cells in only 5 min at room temperature with high specificity and sensitivity. |
