| 초록 |
An on-line solid phase extraction (SPE) method was developed for the simultaneous screening of cefazolin sodium and cefotaxime sodium in human urine. A short weak anion-exchange monolithic column was prepared and used as the selective extraction sorbent. During the on-line SPE process, most matrices in the urine samples were removed rapidly while cefazolin sodium and cefotaxime sodium were effectively trapped in this monolithic column. Reversed-phase high performance liquid chromatography (HPLC) was performed on a C18 column with ultraviolet detection at 254 nm. The mobile phase consisted of methanol-0.005 mol/L phosphate (21/79, v/v, pH=5.0) and the flow rate was set to 0.7 mL/min. The result shows that the on-line SPE-HPLC method is suitable for the simultaneously monitoring of cefazolin sodium and cefotaxime sodium in human urine samples because it avoids tedious pretreatments and provides a fast, economical and reproducible method for assaying trace drugs in biological samples. |
