| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Production of biodegradable plastics, P(3HB-co-3HP) from methane |
| 초록 |
Polyhydroxybutyrate (PHB) with its biodegradable and biocompatible characters is an attractive polymer. However, its application is limited due to the stiffness and brittleness. Co-polymerisation of 3-hydroxybutyrate (3HB) with another monomer is an alternative to generate biopolymers with more desirable properties. In this study, the pathway for production of poly(3-hydroxybutyrate-co-3-hydroxypropionate) (P(3HB-co-3HP)) in a type II methanotroph was constructed by combining native PHB pathway and 3HP production pathway with the expression of 3HP-CoA synthetase (3HPCS) and PHA synthase. The genes encoding 3HPCS and the PHA synthesis pathway were screened to discover the efficient enzymes for condensing 3HB-CoA and 3HP-CoA into P(3HB-co-3HP). The best recombinant strain OB3b-MCRM-3S was found to synthesize P(3HB-co-3HP) from methane with 9% P3HP in 0.75 g/L CDW. This is the first study reported on production of biodegradable plastics using methane as the sole carbon substrate. |
| 저자 |
이은열, Diep Thi Ngoc Nguyen, 이옥경
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| 소속 |
경희대 |
| 키워드 |
type II methanotroph; polyhydroxybutyrate; P(3HB-co-3HP); methane
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| E-Mail |
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