| 초록 |
Here, we report the construction of a novel detection platform comprising of two units: 1) magnetic beads separately conjugated with multiple capturing antibodies against four different high-risk pathogens for simple and rapid isolation, and 2) genetically engineered apoferritin nanoparticles conjugated with multiple quantum dots and detection antibodies against four different high-risk pathogens for signal amplification. For each high-risk pathogen, we demonstrated at least 10-fold increase in sensitivity compared to traditional lateral flow devices that utilize enzyme-based detection methods. Multiplexed detection of high-risk pathogens in a sample was also successful using the nano-constructs harboring dye molecules with fluorescence at different wavelengths. We ultimately envision the use of this novel nano-probe detection platform in future applications that require highly sensitive on-site detection of high-risk pathogens. |
