| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
약물 및 유전자 전달용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Enhanced intracellular delivery and gene silencing by tripodal interfering RNAs |
| 초록 |
Recent progress in the development of functional gene silencing RNA molecules such as siRNA and antagomir has provided promising therapeutic modality by inhibiting specific mRNA-coding genes and microRNAs (miRNAs) involved in disease mechanism. Often, inhibition of multiple target genes is needed to combat against viral infection or cancer, to suppress the escape mutation and to achieve synergistic anti-tumor effects. Here, we report a chemically synthesized, multiple-target gene silencing RNA structures based upon branched, tripodal interfering RNAs (termed tiRNAs). The tiRNAs could simultaneously silence up to three different mRNAs or miRNAs by harboring three siRNA or antagomiR units. In addition to the multiple gene silencing, tiRNAs complexed with cationic delivery reagents showed enhanced intracellular delivery and gene silencing activities, compared with conventional siRNAs or antagomiRs. Therefore, we believe that the tsiRNA structures could become powerful tools for the efficient combinatorial RNAi strategy. |
| 저자 |
Chan Il Chang1, Pooja Dua2, Soyoun Kim3, Dong-ki Lee4
|
| 소속 |
11Global Research Laboratory for RNAi Medicine, 2Department of Chemistry and BK21 School of Chemical Materials Science, 3Sungkyunkwan Univ., 4Global Research Laboratory for RNAi Medicine |
| 키워드 |
a |
| E-Mail |
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