| 초록 |
Small regulatory RNA (sRNA) is the gene expression regulatory system in bacteria. We developed synthetic small regulatory RNAs. The target binding sequence of MicC, one of the sRNAs in Escherichia coli, was substituted to translation initiation sequence of our target genes. Using DsRed2, a fluorescence protein, we found that MicC scaffold based synthetic sRNA is repressed DsRed2 expression. Synthetic sRNAs can be used for metabolic engineering. Using synthetic sRNAs for the knockdown of 4 candidate genes in 14 different strains, we isolated a tyrosine producer producing 2 g per liter of tyrosine. Using 130 synthetic sRNAs, knockdown targets which show substantial increases in cadaverine production were identified. Repression of murE led to a 55% increase in cadaverine production compared to the base strain. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012M1A2A2026556); the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea] |
