| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
A novel biodegradable branched poly(ester amine)s based on glycerol dimethacrylate and low molecular weight polyethylenimine as a gene carrier |
| 초록 |
Gene therapy is a new approach in the treatment of numerous diseases caused by damage or lack of certain genes. Cationic polymers are very promising tools for gene delivery into different cell types, but need to be improved in certain area for success. Polyethylenimine (PEI) is a non-degradable gene carrier with high transfection efficiency and high toxicity as well. We synthesized low cytotoxic biodegradable poly (ester amine)s, as a novel gene carrier. The copolymers were synthesized using low molecular weight branched PEI (1.2 KDa) with a hydrophilic, biodegradable glycerol dimethacrylate through Michael addition as per our previous reports. The complexation of polymer/DNA was characterized for complex forming ability, particle size and morphology. Cytotoxicity and transfection efficiency of copolymers was determined on cultured HeLa, HepG2, and 293T. In vitro transfection assay and cytotoxicity of degradable poly (ester amine)s was quite comparable to that of PEI. |
| 저자 |
로히다스1, 유미경1, 강호림1, 다난자이1, 김유경1, 조명행1, 나재운2, 조종수1
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| 소속 |
1서울대, 2순천대 |
| 키워드 |
poly(ester amine); polyethylenimine; glycerol; biodegradable
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| E-Mail |
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