| 초록 |
Brain plaque deposition in the form of beta amyloid (Aβ) is a pathological hallmark of Alzheimer’s disease (AD). Furthermore, the increment of Aβ deposition is associated with an isoform of Apolipoprotein E4, a dominant genetic risk factor of AD. Here, we present a nanoplasmonic biosensor to detect the dynamics of ApoE4-mediated Aβ aggregation. Based on localized surface plasmon resonance (LSPR) spectra, the rate of Aβ42 aggregation increases more rapidly than that of Aβ40. In this sensor, ApoE4 is exploited as Aβ42 detector and plasmon resonance promoter. In addition, a detection limit of Aβ42 at 50 μM can be obtained corresponding to the LSPR-peak shift of 12.5 nm. This is the first biomimetic platform for measurements of Aβ aggregation in biological conditions, which can be used to investigate AD directly in the future. |
