| 학회 |
한국재료학회 |
| 학술대회 |
2010년 봄 (05/13 ~ 05/14, 삼척 팰리스 호텔) |
| 권호 |
16권 1호 |
| 발표분야 |
D. Health and Biological Materials(생체 및 의용재료) |
| 제목 |
Recruitment of binding proteins in recognizing biomaterials by Toll like receptors. |
| 초록 |
Acute and chronic inflammation induced by biomaterials is the main cause of implant failure. Due to immunological complications between implanted materials and monocyte/macrophage (M/M cells) into the surrounding bone microenvironment, several inflammatory molecules become activated and trigger the inflammation. Recently Toll Like Receptor (TLR) has been reported to be involved in cytokine production by the macrophage cells activated by biomaterial particles. However, the mechanism behind such kind of inflammatory reaction is still poorly understood. We, therefore, in this study tried to evaluate and understand in vitro how TLR participates in the bio-molecular mechanism of inflammation. The productions of TNF-α, IL-1β and IL-6 by macrophage were assayed by enzyme linked immunosorbent assay (ELISA) and their mRNA expression was performed by RT-PCR. The binding protein for TLR and nuclear factor κB (NF-κB) was also identified by western blot method. Moreover, we also assayed the ALP levels and MMP productions by macrophage once activated by biomaterials. |
| 저자 |
M. Anirban Jyoti1, Ho-Yeon Song1, Park Min Ju1, Kim Young Hee1, In-Seon Byun1, Byong-Taek Lee2
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| 소속 |
1Department of Immunology, 2Department of biomedical engineering and materials |
| 키워드 |
inflammation; biomaterials; Toll Like Receptor; cytokine
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| E-Mail |
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