| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
A Study of Proliferation and Morphology of Retinal Pigment Epithelial Cell on SIS/PLGA Film |
| 초록 |
Porcine small intestine submucosa(SIS) is accellular tissue and widely used as a biomaterials without immunorejection responses. Poly(lactic-co-glycolic acid)(PLGA) is a biodegradable synthetic polymer with acceptable mechanical strength and well-controlled degradation rate. RPEs were isolated from retina of rabbit. We fabricated SIS/PLGA films using 20 wt% of SIS. We measured MTT to confirm cellular of cell number attached on film at 1, 3, 7, and 14 days. Morphology of cellular adhesion on films was confirmed by scanning electron microscope (SEM) at 1, 3, and 7 days. RT-PCR was conducted to confirm mRNA expression of RPE65 as RPEs marker and expression of cytokeratin, RPE65 were determined by Immunofluorescence staining. These results suggest that SIS provides suitable surface to RPEs. This research was supported by WCU (R31-20029) and SCRC (SC4100). |
| 저자 |
조은혜, 김수진, 전나리, 김초민, 김순희, 성경열, 이동원, 이종문, 강길선
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| 소속 |
전북대 |
| 키워드 |
retinal pigment epithelial; SIS film; PLGA
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| E-Mail |
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