| 초록 |
We have synthesized nitrilotriacetic acid end-functionalized polystyrene (NTA-PS) for the controlled bioconjugation with histidine-tagged green fluorescence proteins (His6-GFP). NTA-PS was prepared using initiators containing t-butyl protected NTA moiety via atom transfer radical polymerization (ATRP) of styrene; the protected t-butyl group was subsequently removed at the α-chain end of polystyrene. The structure of NTA-PS was characterized using 1H NMR, 13C NMR, and GPC. NTA chain ends of the polystyrenes were complexed with Ni2+ to produce Ni-NTA-PS, of which the specific binding properties were studied by forming spherical aggregates with His6-GFP in aqueous phase. The reversible association of His6-GFP with polystyrene spherical aggregates (with Ni2+) was controlled with imidazole and monitored with fluorescence microscope. Again, Ni-NTA-PS produced well defined micelles with His6-GFP in water/DMF (DMF 4 vol. %) and the size of micelles decreased when excess imidazole was added. |
