| 학회 | 한국화학공학회 |
| 학술대회 | 2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 | 16권 2호, p.2427 |
| 발표분야 | 대사공학 신기술 동향 심포지엄 |
| 제목 | Engineering Corynebacterium glutamicum for biotransformation of cyclohexanone derivatives |
| 초록 | Corynebacterium glutamicum was developed as a biocatalyst for biotransformation of cyclohexanone derivatives. C. glutamicum ATCC13032 was engineered to express chnB the gene of cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB 9871. The chnB gene was functionally expressed, enabling the whole-cell activity of the recombinant of over 70 U/g CDW. The high CHMO activity, high NADPH regeneration capacity, and high tolerance against organic solvents of the recombinant strains allowed to produce ε-caprolactone from cyclohexanone at the specific and volumetric productivity of0.12 g/g dry cells/h and 2.3 g/L/h, respectively, resulting in a final product concentration of 16.0 g/L. The recombinant C. glutamicum was further engineered to increase the reaction activity; the cell wall permeability toward lipophilic organic substrates was increased via modifying the structure of cell wall arabinogalactan and mycolate layers. The engineered C. gltutamicum cells showed the higher oxygenation activity of cyclohexanone derivatives (e.g., ethyl 2-cyclohexanoneacetate, 2-(2’-acetoxyethyl)cyclohexanone) as compared to the wild type based biocatalysts. |
| 저자 | 윤지영, 이정은, 박진병 |
| 소속 | 이화여자대 |
| 키워드 | Biotransformation; Corynebacterium glutamicum; Cyclohexanone monooxygenase; metabolic engineering |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
