| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.2024 |
| 발표분야 |
재료 |
| 제목 |
Enhanced biocatalytic activity of alkaline phosphatase immobilized on gold nanoparticles capped silica nanosphere |
| 초록 |
Gold nanoparticles are known to bind enzymes, but reuse characteristics of the gold nano-enzyme bioconjugates has hitherto been poor.In the present study was synthesis of amine functionalized silica nanoparticles by O/W microemulsion method and it was coupled with 18 nm synthesized gold nanoparticles. After that the prepared gold nanoparticles capped silica were conjugated with enzyme alkaline phophatase. A highlight of the new biocatalyst wherein the enzyme is supported by a more massive biocompatible surface is the ease with which separation from the reaction medium may be achieved by simple sedimentation. The prepared enzyme immobilized Au capped silica nanoparticles and free enzymes biocatalytic activity were analyzed at different pH and temperature and storage ability and reusability were also analyzed in order to investigate the efficiency of nano biocatalyst. |
| 저자 |
Chinnasamy Thiruppathi Raja1, Mari Vinoba1, 조완택2, 정순관2, Muthukaruppan Alagar1
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| 소속 |
1Anna Univ. Chennai, 2한국에너지기술(연) |
| 키워드 |
Silica nanosphere; Gold nanoparticles; ALP; biocatalyst
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| E-Mail |
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| 원문파일 |
초록 보기 |
