| 초록 |
The rapid and sensitive detection of bacterial pathogens is crucial for improving patient care by subsequent treatment with the appropriate antibiotics and preventing spread of the disease. Currently, culture-based assays or staining and microscopy remains the gold standard in the clinic, despite their long procedure times (several days), low sensitivity, and poor specificity. We have investigated various molecular labeling approaches which can be robustly applied in clinical settings for rapid and sensitive detection of bacterial pathogens. We first developed a nanoparticle labeling strategy to ubiquitously detect Gram-positive bacteria using a miniaturized nuclear magnetic resonance device (μNMR). We used small molecule antibiotics (vancomycin and daptomycin) and the Gram stain as targeting ligands, in a two-step bioorthogonal procedure, which allowed rapid and efficient labeling. To specifically identify each type of pathogen, we developed a nanoparticle hybridization technology based on detection of bacterial 16S rRNA sequences (Magneto-DNA nanoparticle system). The magneto-DNA technology allowed rapid and target-specific detection of different types of pathogenic bacteria with extremely high sensitivity in nine clinical specimens. In sum, the molecular sensing strategies based on nanoparticle labeling introduce robust and sensitive platforms for detecting various pathogens and diagnosis of infected patients. |
