| 초록 |
Lingnocelluloseic biomass as feedstock for ethanol production mainly consists of cellulose and hemicellulose. Cellobiose dehydrogenase (CDH) is an extracellular enzyme produced by various wood-degrading fungi. It oxidises cellobiose from lignocellulose to fermentable sugars by a ping-pong mechanism using a wide spectrum of electron acceptors including quinones, phenoxyradicals, Fe3+, Cu2+ and triiodide ion. In this study, new strain of Pichia stipitis was developed by overexpressing CDH gene from Phanerochate chrysosporium under the control of methanol inducible alcohol oxidase(AOXI) promoter. The maxiumum amount of ethanol produced was 5g/L using 50 g/L cellobiose as a carborn source. |
