초록 |
Mannheimia succiniciproducens MBEL55E was isolated from Korean bovine rumen and is maintained at Department of Chemical and Biomolecular Engineering, KAIST. (Daejon, South Korea). It has an ability to produce a large amount of succinic acid from various carbon sources under anaerobic conditions in presence of CO2. The levels of CO2 in the medium have known to be very important factor which affects on the growth of the succinic acid-producing bacteria. Therefore, the effect of CO2 concentration on cell growth and product formation was studied in batch fermentations of M. succiniciproducens MBEL55E. NaHCO3, MgCO3 and CaCO3 were supplied as a donor of CO2 in the medium, respectively. The supplementation of these chemicals enhanced the production of succinic acid and cell growth. It means that NaHCO3, MgCO3 and CaCO3 can act as a donor of CO2. The most effective nutrient was NaHCO3. |