| 초록 |
Controlling release of growth factors from hydrogels has been challenging because encapsulated proteins often showed initial burst release profiles due to fast diffusion process. Heparin enhances a half-life and activity of bFGF, also, protects bFGF from inactivation by acid and heat. In this study, biodegradable hydrogel (22%, w/w) composed of Di-acrylated Pluronic F127 and glycidyl methaacrylated chitooligosaccharide (COS) (0, 20, and 50%, w/w) was prepared in an aim to control release of bFGF. A photo-initiator, Irgacure2959 (0.01%, w/w), was added to the polymer mixture. In order to measure mechanical property of the hydrogel, the hydrogels were monitored using a rotating rheometer. The amount of released rhEGF from the hydrogels was quantitatively determined by ELISA. Mechanical properties of the hydrogels were improved by increasing a content of COS. The chemically-crosslinked hydrogels containing bFGF/heparin can be a superior candidate for tissue engineering scaffold. |
