| 초록 |
Successful immobilization of biological materials by chemical bonding on various solid substrates often requires carefully designed coupling layer, which links biological materials to the solid substrates. It has been well known that there are many factors affecting immobilization efficiency including surface concentration, spacing between anchoring functional groups, defect sites in the coupling layer and the size of the immobilized material. Since many of the coupling layers have been constructed by self-assembly technique, it has been shown that to control of the surface concentration and spacing of the anchoring groups is quite difficult. In this study, we attempt to control surface concentration and spacing of the anchoring group by changing mole ratio of coupling agents in the spreading solution for construction of Langmuir monolayer at the air-water interface. By introducing surface reactive groups at one end of the coupling agent, the LB monolayer transferred onto the solid substrate transformed to a SAM under carefully controlled condition. Amphiphilic thiols, calix[4]arene, and thiobenzene derivatives were used to construct stable Langmuir monolayers at the air-water interface and subsequently convert to SAMs after transferred on the solid substrates. Structure and surface concentration of those LB converted SAMs were investigated by FT-IR spectroscopy and electroanalytical techniques. Immobilization efficiency and biological activity of immobilized protein A, and hIgG were monitored surface plasmon resonance and surface acoustic wave detection techniques. |
