| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 봄 (04/20 ~ 04/21, 대구 인터불고 호텔) |
| 권호 |
12권 1호, p.394 |
| 발표분야 |
생물화공 |
| 제목 |
Development of the Gold Binding Polypeptide-Mediated Surface Plasmon Resonance Sensor Chip |
| 초록 |
The gold binding polypeptide (GBP)-mediated fusion proteins, which were the GBP fused to the N-terminal end of enhanced green fluorescent protein (EGFP) or core streptavidin (cSA) gene of Streptomyces avidinii, were allowed to be specifically immobilized on the bare gold surface by combining a fusion protein technique and microcontact printing (μCP) method. This platform enables the study of protein-protein and DNA-DNA interactions. The successful nanopattern generation and morphology of the fusion proteins bound onto the gold (Au) nanopatterned structures were visualized by Atomic force microscopy (AFM) and characterized by surface plasmon resonance (SPR) imaging. This simple and highly specific immobilization method would be useful for a wide variety of applications such being the use in biotechnological sensors and biochips. [Our work described in this work was supported by the KOSEF through the Center for Ultramicrochemical Process Systems and the R&D program for Regional Development, which is sponsored by the MOCIE] |
| 저자 |
최종현1, 박태정1, 이석재1, 박종필1, 고성호2, 이상엽1, 구수진3
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| 소속 |
1한국과학기술원 생명화학공학과, 2나노종합팹센터, 3(주)케이맥 |
| 키워드 |
SPR; GBP
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| E-Mail |
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| 원문파일 |
초록 보기 |
