| 초록 |
Carbonic anhydrase (CA) is a zinc containing metalloenzyme catalyzing the reversible hydration of carbon dioxide (CO2 + H2O→HCO3-). CA is important in biological systems because the uncatalysed interconversion betweenCO2 and HCO3- is slow.Its high efficiency catalysis is fundamental to many biological processes, such as photosynthesis, respiration.Also ithas practical use in CO2 fixation. In this study, we developed multiple localization-controlled biocatalyst which can express CA efficiently.At first, we built up vector system which can express CA of synechocystis PCC6803&Escherichia coli K12 at cytoplasm and periplasm and excuted cloning. ( expressing CA of synechocystis PCC6803 at cytoplasm, periplasm and CA of E.coli K12 at cytoplasm, periplasm) Next, cloned recombinant vector was transformed BL21(DE3). Expressed protein(CA) was checked as western blotting. At the end, we could confirm CA expression at different vector system. |
