| 초록 |
Recently, biomaterials play a critical role in the engineering of new functional tissues for the replacement of lost or malfunctioning tissues. They provide a temporary scaffolding to guide new tissue growth and organization. Among them of interest to us is acellular collagen-based matrices as scaffolds. The drawback of acellular matrices include durability, immunogenicity and calcification. In this study, to overcome these problems acellular matrix of bovine pericardium(BP) was chemical modified by the direct coupling of L-arginine/heparin containing amino groups, after glutaraldehyde(GA) cross-linking to investigate the effects of L-arginine/heparin coupling on calcification of BP. Arginine promotes angiogenesis, wound healing and cell division. Nitric oxide(NO) formed L-arginine also has important effects on cell growth and interactions of circulatory blood cells with the vessel wall. Heparin is known to help the inhibition of smooth muscle cell proliferation and release of bFGF from the ECM. This may alter the permeability to ions necessary for growth, change confirmation of molecules to which it binds of affect cell volume. Chemical modified acellular matrix of BP was evaluated for thermal stability by DSC, digestion resistance to collagenase enzyme, mechanical properties, cell culture and in vivo calcification, respectively.
|
