| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.92 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Preparation of Biodegradable Polyethylenimine-co- Poloxamer as a Gene Carrier |
| 초록 | The fields of non-viral gene therapy are rapidly gaining increased interest owing to the potential advantages of these non-viral carriers from view points of their safety, production, and versatility. In this study, reaction of the polyethylenimine(PEI) to a poloxamer macromer as a nonionic water-soluble polymer was performed to get a non-toxic and biodegradable gene carrier. The molecular weight and degradation of the PEI-co-Poloxamer (PEI-POL) were determined using GPC. The PEI-POL/DNA complex was confirmed using agarose gel electrophoresis and DNase I experiment. The sizes and zeta potentials of the complex were measured by electrophoretic light scattering. The cytotoxicity of PEI-POL in 293T, A549 and HepG2 cells was evaluated. The PEI-POL/DNA complex was transfected into 293T, A549 and HepG2 cells. Fig. 1 shows representative transfection efficiency of PEI-co-POL/DNA complexes on A549 according to the concentration of poloxamer concentration to PEI. The results indicated that the transfection efficiency of the PEI-co-POL was higher than that of PEI 25 K as the control and the transfection efficiency of it was decreased with an increase of poloxamer content in the copolymer owing to increased hydrophilicity. Fig.1. Transfection efficiency of PEI-co-POL/DNA complexes on A549 |
| 저자 | 조종수1, 국승은1, 나재운2, 박태관3 |
| 소속 | 1서울대, 2순천대, 3한국과학기술원 |
| 키워드 | non-viral gene carrier; biodegradable; polyethylenimine; poloxame |
