| 학회 | 한국화학공학회 |
| 학술대회 | 2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 | 13권 2호, p.1685 |
| 발표분야 | 생물화공 |
| 제목 | Effect of Target Nucleotide Length on Hybridization for Microfluidic DNA Microarray |
| 초록 | On a microfluidic platform, length of target nucleotide is a deterministic factor in behavior of target:probe nucleotide hybridization. DNA hybridization assays are widely used for biomarker detection for both research as well as clinical applications. In this study, three target DNA oligo-nucleotides having different lengths, 18-mer, 38-mer, and 42-mer, were examined with re-circulating flow on a same 18-mer probe immobilized in polyacrylamide gel pad. Re-circulating flow is adopted to overcome extremely slow diffusion of target DNA in a solution without mixing. Depending on the lengths of oligo-nucleotides, their hybridization and melting profiles with temperature were varied significantly, which reflect that extra-lengthy nucleotides were dynamically influenced by re-circulating flows. By comparison of three target nucleotides, relationships between flow and hybridization could be obtained. Furthermore, results could give a basic understanding for an optimal level of fragmentation and labeling of target nucleotides with ribosomal RNA. Our technology could have far-reaching fundamental researches in micro-fluidic dynamics or microarray study. |
| 저자 | 이현호1, Paul Yager2 |
| 소속 | 1명지대, 2Dept. of Bioengineering |
| 키워드 | DNA Microarray; Nucleotide; Hybridization |
| 원문파일 | 초록 보기 |
