| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Intracellular delivery and localization of cationic liposomes containing PEI-DSPE for intratumoral injection of chemotherapeutic agent |
| 초록 |
PEI-DSPE-incorproated liposomes (PD-liposomes) were prepared for intratumoral injection of chemotherapeutic agent. The PEI was directly conjugated with phospholipid such as DSPE via amide linkage using EDC and NHS. The PD-liposomes size was 130 ± 10 nm. Additionally, the zeta potential of the PD-liposomes increased from -25 mV to 30 mV by incorporation of cationic PEI on the liposomal membrane. Transfection efficiency of the PD-liposomes to tumor cell was higher than that of the control liposomes, and this result may be explained by electrostatic interaction between partially negative tumor cell and positive PD-liposomes. Notably, localization of the PD-liposomes at tumor tissue was observed by fluorescence microscopy and the PD-liposomes showed higher localization than the control liposomes and the free calcein. In conclusion, the PD-liposomes are potent carriers to enhance cell transfection and intratumoral localization of chemotherapeutic agent. |
| 저자 |
황태원1, 김태형2, 한희동1, 김문석1, 성하수1, 김중현3, 신병철1
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| 소속 |
1한국화학(연), 2충남대, 3연세대 |
| 키워드 |
temperature-sensitive liposomes; thermosensitive polymer; hyperthermia
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| E-Mail |
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