| 초록 |
Phospholipase D is an enzyme that occurs ubiquitously in various organisms including mammals, plants, yeast and bacteria. We have screened PLD-producing Actinomycetes species, and evaluated several PLDs in culture supernatants. Three Actinomycetes strain, Streptomyces Cs57 and S. Cs 528, Cs 684 release high activity of PLD in the medium. Subsequently, three enzymes were purified from the culture medium and one PLD gene was cloned from S. CS 684. We are developing, for the first time, an efficient expression system for the active PLD and industrial phospholipid synthesis system. The use of PLD gene from extreme microorganisms will improve the phospholipid synthesis. |
