| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Osteogenic potential on the manufacturing method of PLGA scaffold for Tissue engineered bone |
| 초록 |
In this study, three types of PLGA scaffolds were prepared and confirmed effect on method of fabrication for bone tissue engineering applications. We fabricated prepared scaffolds by solvent casting/salt-leaching, ice particle-leaching and gas-forming/salt-leaching method. Proliferation of BMSCs in PLGA scaffolds manufactured by various methods was analyzed using MTT assay. Also, we have characterized the osteogenic potential of BMSCs in scaffolds by ALP, RT-PCR. The biological activity and ALP activity of BMSCs in the scaffolds was the highest in the scaffolds using solvent casting/salt-leaching method. BMSCs in scaffolds were stimulated by osteogenic medium and characterized by Masson’s trichrome and von Kossa staining. We could observe collagen and calcification over broad region in solvent casting/salt-leaching scaffolds.We concluded the manufacture method of scaffold affect to differentiation of BMSCs and solvent casting/salt-leaching is useful for osteogenesis of BMSCs. |
| 저자 |
소정원1, 김순희1, 백미옥1, 임지예1, 최진희1, 정수현1, 홍현혜1, 강길선1, 이해방2
|
| 소속 |
1전북대, 2한국화학(연) |
| 키워드 |
PLGA 지지체 |
| E-Mail |
|
