| 초록 |
Silk fibroin of silkworms has been widely studied as biomaterials. The degradation behavior of silk biomaterials is obviously important for medical applications. But the study about long- term result is few in vivo. In this work, we investigated the degradation behavior of silk fibroin membrane in vitro and in vivo. In vitro assay, we observed degradation of silk membrane in PBS, culture media and enzyme (protease K) solution. In solution with protease K, 80% of silk membranes were degraded within 10 days. Silk membranes presented no cytotoxicity in L929 cells and rat tissue. In order to investigate degradation of silk membrane in vivo, silk membrane implanted subcutaneous in rats and were harvested after surgery until 19 months. SEM, histological analysis of silk membrane explants showed that silk membrane broken in several pieces from 16 months. In conclusion, the results indicated that silk membrane is a good biocompatible and has a long degradation time as biomaterials. |
