| 초록 |
Low molecular and water-soluble chitosan (LMWSC) with a free-amine group was prepared by the novel salts removal method described in this study. A molecular weight (Mw) and degree of deacetylation (DDA) of LMWSC were determined by viscometer and Kina titration, resulting in 18,579 Daltons and 93 % DDA, respectively. In the FT-IR, 1H-NMR, and 13C-NMR spectra, the absorption band by carboxyl group derived from lactic acid and the impurities formed in the enzymatic process disappeared or were significantly lower than that of the control chitosan. Also, by the 1H-NMR, and 13C-NMR spectra, it was identified that the empirical value for the area ratio of proton and carbon corresponds nearly to its theoretical values. Through MALDI-TOF mass spectrum analysis, the difference in the two adjacent peaks was calculated 161. |
