| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.377 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Animal Tests using Small Intestinal Submucosa Sponges for Wound Healing |
| 초록 | Small intestinal submucosa (SIS) is an acellular biomaterial usually derived from the jejunum of pig. SIS consists predominantly (〉90%) of collagens typesⅠ and Ⅲ similar to the dermal component of skin. Other extracellular matrix (ECM) components of SIS consist fibronectin (FN), chondroitin sulfates (A and B), heparin, heparan sulfate, hyaluronic acid, and cytokines, such as basic fibroblast growth factor (FGF-2) and transforming growth factor-β (TGF-β). These components are known to be important for tissue remodeling and wound healing, especially in full-thickness skin defects1. Preparation of SIS sponges refer to the literature2. Sprague-Dawley (SD) rats (180～200 g) of female used and dorsal hair removed. After anesthetized disinfection of the skin with potadine, a full thickness wound of 3 cm in diameter was prepared by excising the dorsal skin of a rat. The excised wound was covered with SIS sponges. Then, a sterilized elastic band (10 ㎝, Tegaderm, 3M Health Care, Canada) was employed to wrap the sponge and sutured to the sponge to the dorsum of a rat. On the postoperative weeks 1, 2 and 3, a skin wound tissue was biopsied from the central regions of the wound in a strip of about 3 by 3 cm. Then, H&E staining was used for wound healing process evaluation. Based on the preliminary histological results at the 1-3 weeks postoperative days, the EDC crossliked SIS sponges showed better wound healing effect in view of a rapid proliferation of fibroblast and epidermal cells in the wound of a SD rat and a significant reduction of infectious cells after 3 weeks, compared with the non-dressing, and the gauze dressing. Reference 1. K. Lindberg, and S. F. Badylak, 27, 254 (2001). 2. H. W. Shin, S. H. Kim, J. W. Jang, M. S. Kim, S. H. Cho, H. B. Lee, and GS Khang, 28, 194 (2004). |
| 저자 | 신혜원1, 홍금덕1, 김선화1, 강길선1, 이해방2, 김문석2, 조선행2, 이혜경3 |
| 소속 | 1전북대, 2한국화학(연), 3대전 가톨릭병원 임상병리과 |
| 키워드 | small intestinal submucosa; wound dressing; histology; in vivo |
