| 초록 |
In recent years, some synthetic bioadhesive oligopeptides such as Arg-Gly-Asp (RGD) and Arg-Glu-Asp-Val (REDV) have been immobilized into polymeric scaffolds to improve specific cell attachment. The interactions between the scaffold surfaces and specific cells play an important role in tissue engineering applications. In this study, non-porous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The films with hydrophobic surfaces were activated by using plasma treatment to prepare hydrophilic ones. Some carboxylic acids on the film surfaces were obtained by acrylic acid grafting. The resulting carboxyl groups were readily reacted in mild aqueous conditions with GRGD and Gly-Arg-Asp-Gly (GRDG) control as peptide ligands for promoting specific cell adhesion. The surface properties of the modified PLLA films were analyzed by ATR-FTIR, ESCA, SEM, and contact angles. In addition, the adhesion and proliferation of the cells on some peptide ligands-grafted films were evaluated. |
