| 초록 |
Hearing loss is a major public health problem, and its treatment with traditional therapy strategies is often unsuccessful due to limited drug access in the cochlea cell. In the previous study, we synthesized poly(2-hydroxyethyl aspartamide)(PHEA) grafted with octadecyl amine(C18)(PHEA-g-C18) to apply for drug delivery into ear. However,the low biomembrane permeability of the polymer was generally considered to be limiting factor in successful delivery of the cochlea. In this study, we use a cell penetrate peptide(CPP), Arg8, to penetrate a round window membrane(RWM) at middle ear. The size of the CPP nanoparticles (PHEA-g-C18-Arg8) was below 50nm and the surface charge of that was positively charged. Also in the cell uptake study, the CPP nanoparticle was transfected to HEI-OC1 cell, mucouses membrane cell at middle ear, higher than PHEA-g-C18 nanoparticles. The result represent that there is a charge interaction between the CPP nanoparticle and a round window membrane. These findings indicate that the CPP nanoparticle can be an useful drug carrier to the coclea. |
