| 초록 |
Catechin compounds from Korean and Chinese green tea, and pu-erh, Indian black, Longjing, Tieguanyin, Bamboo, Jasmine, Oolong, Flower, Red teas as potential powers of anticancer and antioxidant components were target materials in this work. After extracting the green tea with water at 50oC for 4 hr, the extract was partitioned with water/chloroform, which was best suited to remove caffeine impurity from the extract. Further, the resulting extract was partitioned water/ethyl acetate to deeply purify the catechin compounds of EGC, (+) C, EC, EGCG and ECG. The extracted samples were analyzed by reversed-phase high performance liquid chromatography(RP-HPLC). The mobile phase was applied the binary system of A(water/acetic acid, 100/0.1, vol.%) and B(acetonitrile/acetic acid, 100/0.1, vol.%) from 90:10(A:B vol.%) to 70:30(A:B vol.%) in a linear gradient time of 30min. The amount of catechin compounds in Chinese green tea was extracted 114.65% more than that from the Korean green tea. Compare various tea, the contents of the five catechin compounds were above the 1.7times of that from green teas than another tea. |
