| 초록 |
Human genome sequencing project has been finished and post-genomic era is already begun. In the wonderland of complete sequences, there is much that genomics cannot do, and so the future belongs to proteomics: the analysis of complete complement of proteins. Now systematic efforts are currently under way to construct protein microarrays for the analysis of protein expression level. Protein-protein interactions have been widely used to functional study of unknown genes or proteins. Now it is considered as a new approach to functional genomics, drug discovery, and insight of cancer and diseases. We are interested in the development and application of techniques for protein microarray, especially for in vitro studies of protein function and for application of biochip in diagnostics. Development of protein chip systems provides a sensitive, multipurpose, effective and easy technology to determine protein interactions to protein, nucleic acid, and ligands. Protein chip could analyze hundreds of proteins in a single experiment, so it is a very effective method to screen proteins as drug targets in cancer and other human diseases. Since technologies of electronics and protein microarray are used in the construction of protein chip, cooperation of KRIBB and ETRI in expert system, is on the way for the effective study of it. We immobilized proteins onto chemically derivatized glass slides coated with gold, or silicone wafer at high spatial densities. The proteins attached covalently to the slide surface yet retained their conformation and ability to interact specifically with other proteins in solution. We will develop protein microarrays for applications such as screening for protein-protein interactions, identifying the substrates of protein kinases and phosphatases, and identifying the protein targets of small molecules. A high-precision robot designed to manufacture complementary DNA microarrays will be used also to spot proteins onto slides. |
