| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
One step purification and immobilization of enzymes using Poly(2-acetamido acrylic acid) PAAA hydrogel |
| 초록 |
We developed one-step purification and immobilization system of enzymes using metal-complexed poly(2-acetamido acrylic acid) (PAAA) hydrogel. Directly from cell lysate, polyhis-tagged recombinant enzymes (Glutamyl aminopeptidase) were purified and immobilized to the PAAA hydrogel via the chelated metal ion affinity interaction. PAAA hydrogel was synthesized using 2-Acetamidoacrylic acid(AAA) and 2,2´-[(1,4-dioxo-1, 4-butanediyl)diimino] bis(2-propenoic acid) in DMSO by potassium persulfate(KPS). For the preparation of the Ni2+ -complexed PAAA hydrogel(Ni2+ -PAAA hydrogel), the PAAA hydrogel was immersed in solution of 0.1M nickel chloride(NiCl2) and then maintained under stirring at room temperature for 24h. In the presentation, the enzyme binding capacity, enzymatic activity, and reuse and storage stability of immobilized enzyme will be discussed. |
| 저자 |
하은주1, 이선구1, 박형순2, 이장우1, 백현종1
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| 소속 |
1부산대, 2Probiond Co. Ltd. |
| 키워드 |
Hydrogel; Enzyme; Purification; Immobilization
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| E-Mail |
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