| 초록 |
The production of value-added chemicals through metabolic engineering was mainly produced from sugar-based carbon sources. However, as economic and environmental concerns of bioprocess have emerged, more attention has been paid to the chemical production from C1 carbon sources. In this study, we have developed the E. coli-based synthetic formatotroph that are able to utilize formate as a carbon source in sugar-free conditions. For formate assimilation, the genes related to the tetrahydrofolate cycle and serine cycle were overexpressed from Methylobacterium extorquens AM1. Additionally, adaptive evolution was carried out until the desired phenotypes emerged to overcome the problems of toxicity and low biomass conversion rate. After conducting the 150th serial subcultures, mutants’s genome was sequenced. Based on sequencing, a few possible mechanisms that might increase the formate utilization were examimed. Finally, the resulting E. coli was engineered to convert formate into ethanol. |
