| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.389 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Polyethylenimine as Gene Delivery Carrier for Various Cells |
| 초록 | To avoid using viral vector systems that may elicit undersirable safety problems, non-viral vector systems are an attractive alternative method to deliver DNA into cells. Poly(ethylenimine) (PEI) has been known as an efficient gene carrier with the highest cationic charge in their structures. PEI are receiving the growing attention as non-viral vectors for gene therapy. PEI as cationic source complexes with anionic phosphate of DNA through electrostatic interaction, resulting in a reduced size of the DNA and an enhanced stability toward DNase. Efficient delivery of foreign DNA into cells is an extremely critical step in gene therapy. In this work, we tried to examine the transfection using for various cells. DNA condensation of PEI was slightly impaired at lower N/P ratio. Their complexes with plasmid DNA were examined for cellular uptake behaviors by using fluorescence microscopy and flow cytometry1. The transfection efficiency in rat muscle-drived stem cells (MDSCs), rat bone marrow stromal cells (BMSCs), NIH/3T3 fibroblast cell line, PC12 and Schwann cells were observed. The transfection efficiency was about 20% although if dependent on a kind of cells. We investigated the cytotoxicity by the MTT assay method. Low cell viability was observed at high N/P charge ratio. We confirm that PEI can served as the efficient gene delivery carriers for various cells. Acknowledgement : This work was supported by Korea Ministry of Commerce, Industry and Energy(N11-A08-1402-05-1-3) Reference 1. Y. J. Park,, J. F. Liang, K. S. Ko, S. W. Kim, and V. G. Yang, J Gene Med, 5, 700 (2003). |
| 저자 | 김선경1, 김선화1, 김문석2, 조선행2, 강길선1, 이해방2 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | gene transfection; PEI |
