| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 봄 (04/05 ~ 04/07, 대전컨벤션센터) |
| 권호 |
42권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
Reactive Polymer Brush-Grafted Particles as Platforms for Protein Immobilization |
| 초록 |
Antibody-antigen precipitation or immunoprecipitation (IP) experiments are routinely performed by biologists to isolate specific antigens and also to identify their interactors from complex protein mixtures. However, the conventional approach involving agarose supports often suffers from highly nonspecific binding and antibody contamination. To overcome these disadvantages, we prepared poly(pentafluorophenyl acrylate) brushes grafted onto silica beads via the SI-RAFT polymerization. Poly(PFPA) is known to have high reactivity with amine groups that offers facile approaches to realize diverse functions starting from the same platforms. These platforms could also circumvent the conventional problems by covalently immobilizing antibodies together with amino-terminated PEG on the brushes. Taking into account their versatility and convenient features of such reactive platforms, we expect that the poly(PFPA) platforms could be utilized as an effective alternative to traditional platforms. |
| 저자 |
손현주1, Sheng Li2, 김유식2, 차국헌1
|
| 소속 |
1서울대, 2KAIST |
| 키워드 |
Polymer Brush; Immunoprecipitation
|
| E-Mail |
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