| 학회 |
한국재료학회 |
| 학술대회 |
2017년 가을 (11/15 ~ 11/17, 경주 현대호텔) |
| 권호 |
23권 2호 |
| 발표분야 |
B. 나노화학/바이오 분과 |
| 제목 |
Haemostatic Evaluation of Thrombin Immobilized Oxidized Cellulose-Silk Fibroin Structure for Surgical Applications |
| 초록 |
The aim of this study is to design and investigate hemostatic activity of thrombin (Th) immobilized TEMPO-oxidized cellulose nanofiber (TOCN)-silk fibroin (SF) scaffolds. Observation of the surface of scaffolds by SEM showed porous structure as well as connections among pores. Porosity of the structures was decreased after immobilization of thrombin on the surface compared to bare TOCN-SF scaffolds. In addition, the in vitro degradation of scaffold was examined for four weeks. The protein adsorption capacity was measured and in vitro thrombin release from scaffold showed that cumulative release of thrombin happen due to immobilization of thrombin on TOCN-SF scaffolds. The scaffolds facilitated the growth of L929 fibroblast cells, where scaffolds with higher silk fibroin content and thrombin immobilization showed higher cell viability. In vivo evaluation of TOCN-Th, TOCN-SF-Th composites were examined using rabbit ear artery model. The results of showed that after immobilization of thrombin, hemostatic activity of each of the scaffolds increased, where blood absorption capacity of the scaffolds reduced. The results of interaction of blood cells/scaffolds demonstrate that RBCs and platelets can strongly interact with hemostatic systems. Thus, it appears that TOCN-SF-Th composite scaffolds can be useful as a novel hemostatic material in clinical application. |
| 저자 |
Anha Afrin Shefa1, 이병택1, Mirana Taz1, Sun Young Lee2
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| 소속 |
1순천향대, 2Division of Environmental Material Engineering |
| 키워드 |
Hemostatic; Thrombin; In Vitro; In Vivo; Blood Cells
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| E-Mail |
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