| 학회 |
한국고분자학회 |
| 학술대회 |
2010년 가을 (10/07 ~ 10/08, 대구 EXCO) |
| 권호 |
35권 2호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Effect of Crosslinked Alginate Films on Proliferation and Phenotype of Corneal Endotherial Cells |
| 초록 |
To evaluate the percentage of endothelial cell damage induced during a surgical technique of Descemet's membrane separation containing healthy endothelium. Alginate is a polysaccharide block copolymer consisting of repeats of -D-mannuronic acid and R-Lguluronic acid monomer. A new method is identified here for producing Ca2+ cross-linked thin films of sodium alginate, using an 0.4, 0.6, and 0.8M CaCl2 solution. Corneal endotherial cells(CEC) were seeded into 1% alginate films. After 1, 3 and 7days of cell seeding, cell proliferation and viability were measured via MTT assay. RT-PCR was conducted to confirm mRNA expression of ColⅤfor CEC marker. We confirmed that cell viability on alginate films of 0.6M were higher than other films. Also, RT-PCR and histology staining results, alginate films of 0.6M showed that the improved gene characteristic of CEC cells. This research was supported by WCU(R31-20029) and Musculoskeletal Bioorgan Center (0405-B001-0204-0006). |
| 저자 |
정수미, 안우영, 이유정, 송정은, 홍동현, 이동원, 강길선
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| 소속 |
전북대 |
| 키워드 |
CEC; Alginate films; crosslinking
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| E-Mail |
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