| 초록 |
Polycaprolactone(PCL) is a non-toxic aliphatic polyester with biodegradability and biocompatibility, which has a potential as a promising bone substitutive material because of its slow degradation properties. Hydroxyapatite(HA) which mimics the natural bone minerals has good osteoconductive properties and poor fabrication properties. Hyaluronan benzyl ester(HyA-B), which can be processed to a great variety of products without altering the biocompatibility and biological activity of hyaluronic acid(HyA), has been found to be suitable substrate to grow a variety of cell types. Therefore, porous biodegradable PCL/HA/HyA-B scaffold was prepared to have the good mechanical and osteoconductive properties, and suitable surface for cell attachment and proliferation. HyA was modified with benzyl bromide to form HyA-B that was soluble in organic solvents. Also, porous biodegradable PCL/HA/HyA-B scaffold was fabricated by a gas-foaming method using a combination of two effervescent salts, ammonium bicarbonate and citric acid. The compressive mechanical strength and morphology of the scaffold were examined. Human MG63 osteoblast was used to investigate the in vitro biocompatibility of the scaffold. The proliferation, morphology, and mitochondrial function of cells cultured on the scaffold were analyzed by PKH26 staining and cell cycle analysis software, SEM, and MTT assay, respectively. |
