| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 |
13권 2호, p.1712 |
| 발표분야 |
생물화공 |
| 제목 |
Specific and sensitive detection of nucleic acids and RNases using gold nanoparticle-RNA-fluorescent dye conjugates |
| 초록 |
Gold nanoparticle (GNP)-based assays have been used to detect biomolecules such as DNA and proteins. These assays rely on the ability of GNPs to undergo colorimetric changes or induce fluorescence quenching of proximal dyes. The highly efficient energy transfer between GNPs and fluorophores provides a basis for improved assay design and multiplexing potential. Here we describe our use of GNPs coated with RNA for the detection of specific DNA sequences and of two RNA metabolizing enzymes, RNase H and RNase A. |
| 저자 |
김중현1, 김성지2, Norbert Reich3
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| 소속 |
1포항공대 정보신소재 (연), 2포항공대 화학과, 3Univ of California |
| 키워드 |
Gold Nanoparticle; Fluoresence Energy Transfer; DNA; RNase H; RNaase H
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| E-Mail |
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| 원문파일 |
초록 보기 |
