| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.400 |
| 발표분야 | 생물화공 |
| 제목 | Xylose-utilization of recombinant S. cerevisiae containing XYL1, XYL2 and XYL3 isolated from Pichia stipitis |
| 초록 | Lignocellulosic biomass is the most abundant source of bioethanol in the world. Saccharomyces cerevisiae is effective for industrial scale production of ethanol, but it cannot utilize xylose as the sole carbon source which are present in high amounts in lignocellulosic biomass. Therefore, many research groups have tried to produce ethanol by introduction of metabolism of xylose fermentation into S. cerevisiae. Pichia stipitis is a good candidate for the development of a recombinant S. cerevisiae for ethanol production through the xylose utilization. Our objective in the present research was to construct the recombinant S.cerevisiae capable of xylose utilization, which contain xylose reductase (XR), xylitol dehydrogenase (XDH) and xylulokinase (XK) from Pichia stipitis. Plasmid pYES2.0 was used for the overexpression of XR, XDH and XK in S. cerevisiae. The ORF of XYL1, XYL2 and XYL3 were isolated from genomic DNA of P. stipitis CBS5773. In addition optimize fermentation for improving ethanol productivity. |
| 저자 | 한지혜1, 박주용1, 강현우2, 정봉우1, 최기욱2, 민지호1 |
| 소속 | 1전북대, 2창해에탄올 |
| 키워드 | bioethanol; xylose reductase; xylitol dehydrogease; xylulokinase |
| 원문파일 | 초록 보기 |
