| 학회 | 한국화학공학회 |
| 학술대회 | 2009년 봄 (04/23 ~ 04/24, 광주 김대중컨벤션센터) |
| 권호 | 15권 1호, p.401 |
| 발표분야 | 생물화공 |
| 제목 | Expression of theXI andXKfrom Clostridium phytofermentans improvesxylose utilizationof recombinant Sacchromyces cerevisiae |
| 초록 | Clostridium phytofermentans has the ability to fermentans broad range of feed-stocks.In C. phytofermentans, xylose isomerase (XI) catalyzes the reversible isomerization of D-xylose to D-xylulose, and xylulokinase (XK) converts D-xylulose to D-xylulose-5-phosphate which enters the pentose phosphate pathway. To study expression of bacterial XI and XK in S. cerevisiae, the XI mutant of this yeast was used as a recipient strain for transformation with the plasmids pYES2 carrying the C. phytofermentans XI gene.This result showed that cells are able to grow with xylose as sole carbon source. It also found that level of ethanol production was increased by fermentation of xylose, comparing with normal S. cerevisiae. It was also showed the more efficient fermentation of xylose to ethanol yield in recombinant S. cerevisiae, comparing a prototypic C. phytofermentans. Recombinant strain of S. cerevisiae engineered for improved xylose utilization are described in this study.This strain harboring XI and XK isolated from C. phytofermentans, shows a significant increase in ethanol productivity with xylose utilization. |
| 저자 | 박주용1, 한지혜1, 강현우2, 정봉우1, 최기욱2, 민지호1 |
| 소속 | 1전북대, 2창해에탄올 |
| 키워드 | bioethanol; xylose isomerase; xylulokinase; Clostridium phytofermentans |
| 원문파일 | 초록 보기 |
