| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 | 28권 2호, p.250 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Effect of dexamethasone and ipriflavone on osteogenic differentiation of muscle-derived stem cells |
| 초록 | Abstract : Ipriflavone, a non-hormonal isoflavone derivative, has been shown to interfere with bone remodeling by inhibiting bone resorption and stimulating bone formation. Muscle-derived stem cells (MDSCs) can be differentiated in culture into osteoblasts, chondrocytes, and even myoblasts. In this study examines and compares the effects of dexamethsone and ipriflavone-dependent in vitro osteogenic differentiation of muscle-dericed stem cells. For osteogenic differentiation, MDSCs were cultured with osteogenic medium included 10-8M dexamethasone, DMEM, 10% FBS, 10-8M vitamin D3, 10mM β-glycerophospahte, 50㎍/㎖ L-ascorbic acid, and 10-8M ipriflavone, DMEM, 10% FBS, 10-8M vitamin D3, 10mM β-glycerophospahte, 50㎍/㎖ L-ascorbic acid. Cellular viability and proliferation were assayed by MTT test, and osteogenic differential cell were analyzed by alkaline phosphate activity, histochemical staining, and RT-PCR. We can be observed cell proliferation of MDSCs increased according to the time and ALP level which ipriflavone group is higher than dexamethasone group. In von Kossa staining, we can observed that ipriflavone group is higher than dexamethasone group by observing calcification. Acknowledgement : This work was supported by MOWH(IMT-2000, 01-PJ11-PG9-01NT00-0011). |
| 저자 | 장지욱1, 양대혁2, 박기숙2, 김은정2, 이상진3, 이봉1, 한창환4, 김문석3, 조선행3, 이해방3, 강길선2 |
| 소속 | 1부경대, 2전북대, 3한국화학(연), 4가톨릭의과대 |
| 키워드 | ipriflavone; dexamethasone; osteogenic differentiation; muscle-derived stem cell |
