| 학회 | 한국고분자학회 |
| 학술대회 | 2003년 봄 (04/11 ~ 04/12, 연세대학교) |
| 권호 | 28권 1호, p.252 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Porous Polymeric Scaffolds |
| 초록 | Articular cartilage once damaged due to traumatic injury or osteoarthritis has a limited capacity for self-repair. Bone marrow derived mesenchymal stem cells (MSCs) are highly proliferative and multipotential cells that have been considered as ideal candidate cells for tissue engineering applications. In this study, we have characterized the chondrogenic potential of rabbit MSCs in porous polymeric scaffolds, PLGA, DBP/PLGA, SIS/PLGA and PGA as three-dimensional constructs to support chondrogenic differentiation following TGF-β1 treatment. In vitro, MSCs were seeded in polymeric scaffolds for 28 days. The chondrogenesis of MSCs-seeded polymer scaffolds culture was assessed by histology and RT-PCR for chondrogenic phenotype. In histological analysis, we confirmed that chondrogenic differentiated rabbit MSCs expressed chondrocyte-like morphologies. Also it was observed that type Ⅱ collagen expressed by RT-PCR. Acknowledgment : This work was supported by MOWH (IMT-2000, 01-PJ11-PG9-01NT00-0011). |
| 저자 | 박기숙;장지욱;김은정;이상진;이봉;한창환;이일우;강길선;이해방 |
| 소속 | 전북대 |
| 키워드 | chondrogenesis; scaffold; stem cell; TGF-β; RT-PCR |
