| 학회 |
한국고분자학회 |
| 학술대회 |
2003년 가을 (10/10 ~ 10/11, 부경대학교) |
| 권호 |
28권 2호, p.91 |
| 발표분야 |
특별 심포지엄 |
| 제목 |
Covalent Attachment of DNA Oligonucleotide and Hybridization on Single-Walled Carbon Nanotube Multilayer Film |
| 초록 |
The immobilization of DNA oligonucleotides on single-walled carbon nanotube (SWNT) multilayer films is a crucial step for any application in the field of DNA microarrays. It determines the efficacy of hybridization and influences the signal strength for the detection. For the immobilization of DNA, we used SWNT multilayer films made from the chemical reaction using the cut SWNTs and the chemical reagents onto an amine-terminated glass. These SWNTs multilayer films were constructed via consecutive condensation reactions that created stacks of SWNT layers linked together with the aid of a linker molecule 4,4’-oxydianiline (ODA) and a condensation reagent. The aminated or carboxylated DNA oligonucleotides were covalently immobilized to the carboxylated or aminated SWNTs multilayer films via amide bonds by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-catalyzed amidation reaction. Absorbance spectrophotometry (UV-vis-NIR) analysis indicated that the surface density of SWNT films increased uniformly according to the number of reaction cycles. Scanning electron microscopy measurements results showed that the SWNT multilayer film thoroughly and uniformly covered the substrate surface. The DNA oligonucleotide probe attached onto SWNT multilayer films and subsequent hybridization has been investigated using X-ray photoelectron spectroscopy and fluorescence-based measurements of DNA hybridization. The resultant DNA-attached SWNT multilayer films were shown to exhibit excellent specificity and chemical stability under the conditions of DNA hybridization. |
| 저자 |
정대환, 김병훈, 고영관, 이상엽, 정희태
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| 소속 |
한국과학기술원 |
| 키워드 |
Single-Walled Carbon Nanotube; Multilayer Film; DNA
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| E-Mail |
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