| 학회 |
한국화학공학회 |
| 학술대회 |
2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 |
21권 1호, p.406 |
| 발표분야 |
생물화공 |
| 제목 |
Phenol production from glucose via metabolically engineered Escherichia coli |
| 초록 |
Microbial production of phenol, a commodity petrochemical, from renewable resources has been restricted due to its toxicity to microbes. Herein, we employed the synthetic regulatory sRNA technology to simultaneously engineer 18 Escherichia coli strains for the production of phenol. Specifically, sRNA-based knock-down of two regulators and overexpression of the tyrosine biosynthetic pathway genes together with tyrosine phenol-lyase in E. coli strains resulted in the production of phenol from glucose. Biphasic fed-batch fermentation using glycerol tributyrate as an extractant of phenol increased the final titer and productivity up to 3.79 g/L and 0.18 g/L/h, respectively. This is the highest titer achieved by microbial fermentation. [This work was supported by the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT & Future Planning through the National Research Foundation of Korea.] |
| 저자 |
Ziwei Luo, 김병진, 박혜권, 나도균, 이상엽
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| 소속 |
KAIST |
| 키워드 |
Phenol; Metabolic engineering; Synthetic sRNA
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| E-Mail |
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| 원문파일 |
초록 보기 |
