| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Versatile method for identification of porcine N-glycan-binding immune cells via solid-phase glycan immobilization |
| 초록 |
For analysis of protein-protein interactions, it is useful to immobilizing glycan and epitope recognition on a solid support. However, identifying glycan-binding cells remains a challenge because there is not enough high-throughput experimental method for the examination of cell-glycan interaction. Here, we describe a tool which enables the screening of glycan-binding cells that specifically recognize the glycan epitopes released from the membrane glycoproteins of cells. Biotinylated pig kidney N-glycans were immobilized on streptavidin-conjugated solid supports and then incubated with human immune cell lines. U937 cells discriminated between the α-galactose (α-Gal) and non-Gal pig N-glycans on the solid support. The method could aid exploration of cell-cell interactions. |
| 저자 |
박한규1, 황철환1, 안다희1, 정윤이1, 송원석2, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
glycan-binding cells; N-glycan; pig kidney; α-galactose
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| E-Mail |
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