| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 가을 (11/08 ~ 11/10, 부산 벡스코(BEXCO)) |
| 권호 |
21권 2호 |
| 발표분야 |
환경에너지_포스터 |
| 제목 |
Immobilization of horseradish peroxidase to an Au nanoparticles decorated on a methionine/graphene biomatrix for the detection of endocrine disruptor bisphenol A |
| 초록 |
In the present study, we employ a straightforward, benign strategy to prepare thiol-functionalized reduced graphene oxide (S-RGO) using methionine as the sulphur source and reducing agent. The immobilization of horseradish peroxidase (HRP) over the AuNPs/S-RGO was developed by incorporating AuNPs on the S-RGO surface. The biosensor shows an excellent amperometric analytical performance with a low detection limit of 2.6× 10-12 M and a linear range of 2.1× 10-11 M to 1.18 × 10-9 M, with the response time ＜ 2 s for BPA. From the results, the apparent Michaelis-Menten constant was calculated as 8.14 nM. Finally, the constructed biosensor was utilized successfully for detecting BPA in tomato juice and milk samples with acceptable results. |
| 저자 |
허윤석1, A.T.Ezhil Vilian2, 한영규2
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| 소속 |
1인하대, 2동국대 |
| 키워드 |
Reduced graphene oxide; Bisphenol A; Horseradish peroxidase
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| E-Mail |
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