| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 | 9권 2호, p.2096 |
| 발표분야 | 생물화공 |
| 제목 | THE SCAFFOLD FOR BONE FORMATION CONCOMITANT WITH IMPROVED ANGIOGENESIS THROUGH 3-DIMENSIONAL CO-CULTURE SYSTEM |
| 초록 | It is well known that endothelial cells play very important roles in differentiation of osteoblasts as well as angiogenesis. In this study, our aim is an understanding that cell-cell interaction and cell-matrix interaction depending on inoculation cell density ratio and implantation period. So, we manufactured scaffolds, which were based on collagen and tricalcium phosphate. These biocompatible and porous scaffolds are suitable to bone regeneration and angiogenesis induction. We obtained osteoblastic cells from HBMSCs (human bone marrow mesenchymal stem cells) and HUVECs (human umbilical vein endothelial cells) from umbilical cord. We found that inoculation cell density ratio was optimal at 1:1 and prolonged implantation period increased calcium deposition. As another result, we confirmed that there were more blood vessels under optimal condition than the other conditions. |
| 저자 | 김만식1, 박정극1, 송계용2, 김영진3, 서영권1, 윤희훈1, 김희숙4 |
| 소속 | 1동국대, 2중앙대, 3(주)라이프-코드, 4삼성제일병원 |
| 키워드 | scaffold; cell culture |
| 원문파일 | 초록 보기 |
